Recombinant Histone 3 antibody (H3K27me3)

Catalog No. ABIN7072680

This anti-Histone 3 antibody is a Rabbit Chimeric antibody detecting Histone 3 in WB, IHC, IF, ELISA, IP and ICC. Suitable for Human.

Quick Overview for Recombinant Histone 3 antibody (H3K27me3) (ABIN7072680)

Target: Histone 3 (H3) (Histone H3 (H3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: BT164

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K27me3

Purpose: Anti-H3K27me3 [BT164], Rabbit IgG, kappa

Specificity: The epitope of BT164 was mapped in the N-terminal tail of histone H3 (27-KSAPAT-32) and included the apoptosis-induced trimethylation of K27.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: BT164 is a mice-derrived lupus antibody that was established by selection on apoptotic chromatin.

Isotype: IgG kappa

Application Details

Application Notes: BT164 recognizes an apoptosis-induced trimethylation of K27 on N-terminal tail of histone H3 which is a target for autoantibodies in patients and mice with SLE (systemic lupus erythematosus) (van Bavel et al., 2011, pmid: 20699234). Consequently, this antibody is recommended for the detection and studying of SLE. ELISA studies demonstrated that BT164 exhibits preferential activity with the apoptotic nucleosomes in comparison with the control nucleosomes (van Bavel et al., 2011, pmid: 20699234). It can be used for the detection of apoptosis in general. Furthermore, this antibody was demonstrated to label cells with methylated histone-3 on K27 via immunofluorescence and it also immunoprecipitated chromatin with such a methylation profile, further demonstrating its ability to specifically recognize markers characteristic of SLE (van Bavel et al., 2011, pmid: 20699234).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Trimethyl-Histone H3 (Lys27), histone H3, H3K27, Trimethylated histone H3, Histone H3 trimethylated at Lysine 27, nucleosome, H3K27ME3, post translational modification, histone post-translational modification